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Image Search Results
Journal: bioRxiv
Article Title: Mgl2 + cDC2 triggering of fungal allergic inflammation depends on a spore induced glycolytic shift fuelled by local availability of glucose
doi: 10.1101/2025.07.13.664342
Figure Lengend Snippet: A ) Mice were exposed to labelled A. fumigatus ( Af ) spores (strain: CEA10, 4 x 10 5 spores) via intranasal (i.n.) transfer, 3h later following CD45 administration i.n. lung tissue was harvested for Precision Cut Lung Slices (PCLS). B ) Representative images show PCLS from top and side views illustrating spores (white), airway CD45 + spore + immune cells (purple) or epithelial cells (green) of Af -exposed mice. C ) Graphs display the number and proportion of spores in airway CD45 + cells, epithelium or in extracellular spaces from PCLS of Af -exposed mice. ROI: region of interest. D ) Naïve mice or mice that were repeatedly exposed to unlabelled Af via intranasal transfer on the indicated time points, were exposed to GFP-expressing Af . Tissues were harvested 3h after the first, sixth or ninth dose of spores (1x, 6x and 9x, respectively). E ) Representative tSNE plots reveal the immune cell populations that acquire spores by unbiased clustering analysis of flow cytometry data from the lung of mice exposed to a single or repeat dose of spores. Graphs display the numbers and percentages of spores acquired by alveolar macrophages (AlvM), interstitial macrophages (IntM), DCs, monocytes, eosinophils and neutrophils from the lung tissue. F ) Representative tSNE plots reveal the DC subsets that acquire spores by unbiased clustering analysis of flow cytometry data from the lung of mice exposed to Af spores. Graphs display the numbers and percentages of spores acquired by DC subsets from the lung tissue. G ) Representative flow cytometry overlay plots identify expression of CD40 and CD86 on spore + and spore - DCs from the lungs of mice exposed to 9 doses of spores. Graphs show the fold change in expression of spore + versus spore - DCs of each DC subset. The 3 DC subsets which acquire the greatest number of spores are shown. A – C ) Number of spores quantified from Region of Interest (ROI) pooled from 3 independent experiments (6-8 ROIs per experiment, 1 mouse per experiment), n = 3 biologically independent animals). D – G ) data are from two independent experiments (n = 6 - 8 biologically independent animals per group). Significance was determined by B ) an unpaired 2-tailed Student’s t-test between indicated groups, E & F ) 1-way ANOVA with Tukey’s multiple-comparison post-hoc test or G ) a paired Student’s t test *P < 0.05, **P<0.01, ***P < 0.001, **** P<0.0001, NS not significant,
Article Snippet: Acquired z-stack images were then analysed by 3D rendering of spores, epithelial and
Techniques: Expressing, Flow Cytometry, Comparison